4O15
The crystal structure of a mutant NAMPT (S165F) in complex with GNE-618
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-01-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97397 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.452, 106.399, 83.630 |
| Unit cell angles | 90.00, 96.83, 90.00 |
Refinement procedure
| Resolution | 46.462 - 1.800 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 96336 | |
| Completeness [%] | 99.3 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 292 | 0.1M Sodium phosphate, pH 8.6, 25% polyethylene glycol 3350, 0.2M NaCl and 1mM compound, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
