4O0P

Crystal Structure of D. radiodurans Bacteriophytochrome Photosensory Core Module in its Dark Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98044
Spacegroup name	P 64
Unit cell lengths	150.280, 150.280, 145.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.650 - 3.800
R-factor	0.2536
Rwork	0.253
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o9c
RMSD bond length	0.006
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.650		3.900
High resolution limit [Å]	3.800	15.000	3.800
Rmerge	0.392	0.147	4.513
Rmeas		0.156	4.725
Number of reflections	18551	310	1362
<I/σ(I)>	4.72	12.48	1.05
Completeness [%]	99.9	96.9	100
Redundancy	10.97	9.6	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	30% PEG 1500, in dark, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K