4NZK

Crystal structure of a DHHW family protein (EUBSIR_00411) from Eubacterium siraeum DSM 15702 at 1.49 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918401,0.979346,0.979261
Spacegroup name	P 61 2 2
Unit cell lengths	102.125, 102.125, 123.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.481 - 1.490
R-factor	0.1264
Rwork	0.125
R-free	0.15770
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.653
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.481	29.481	1.530
High resolution limit [Å]	1.490	6.660	1.490
Rmerge		0.015	0.015
Total number of observations		7605	46602
Number of reflections	62386
<I/σ(I)>	12.5	9.9	0.5
Completeness [%]	100.0	98.9	100
Redundancy	10.3	9.1	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.20M Sodium Iodide, 20.0% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K