4NZF

Crystal structure of Abp-D197A (a GH27-b-L-arabinopyranosidase from Geobacillus stearothermophilus), in complex with arabinose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	107.600, 201.505, 286.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.710 - 2.190
R-factor	0.17098
Rwork	0.168
R-free	0.21888
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nxk
RMSD bond length	0.019
RMSD bond angle	1.988
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.710	2.250
High resolution limit [Å]	2.190	2.190
Number of reflections	268240
Completeness [%]	89.0	76.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.8M Ammonium Sulfate, 0.1M citrate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K