4NZB
NS9283 bound to Ls-AChBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 101 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.726, 140.381, 119.545 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 28.241 - 2.680 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ZDG (chains A-E) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.588 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.241 | 28.241 | 2.820 |
| High resolution limit [Å] | 2.680 | 8.470 | 2.680 |
| Rmerge | 0.041 | 0.379 | |
| Number of reflections | 105283 | ||
| <I/σ(I)> | 11.9 | 14.6 | 2 |
| Completeness [%] | 98.1 | 97.3 | 87.6 |
| Redundancy | 3.6 | 3.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1mM Tris, 0.95M (NH3)2SO4, 4% PEG 400, 0.1M NaCl, 2.5% DMSO, pH 8.0, vapor diffusion, sitting drop, temperature 293K |
