4NYQ
In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 1.2 A resolution of a glycosylated, lipid-binding, lipocalin-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2005-05-10 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.8349 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.333, 33.280, 40.136 |
| Unit cell angles | 99.07, 100.17, 103.70 |
Refinement procedure
| Resolution | 27.450 - 1.200 |
| R-factor | 0.14562 |
| Rwork | 0.143 |
| R-free | 0.19191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nyr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.740 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.450 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 46801 | |
| Completeness [%] | 95.6 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | In-vivo crystallization | 6 | 300 | Spontaneous crystallization inside the midguts of a living viviparous cockroach, pH 6.0, In-vivo crystallization, temperature 300K |
