4NYL
Crystal structure of adalimumab FAB fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 131.420, 47.048, 168.515 |
| Unit cell angles | 90.00, 101.33, 90.00 |
Refinement procedure
| Resolution | 42.030 - 2.800 |
| R-factor | 0.27745 |
| Rwork | 0.275 |
| R-free | 0.35841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.511 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 45174 |
| <I/σ(I)> | 4.96 |
| Completeness [%] | 87.3 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Tris HCl, 20% PEG 3350, 0.2M calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
