4NXY
Crystal Structure of the GNAT domain of S. lividans PAT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.056, 56.711, 74.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.520 - 1.449 |
| R-factor | 0.17845 |
| Rwork | 0.176 |
| R-free | 0.21678 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.441 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.480 |
| High resolution limit [Å] | 1.449 | 1.449 |
| Rmerge | 0.039 | 0.249 |
| Number of reflections | 30540 | |
| <I/σ(I)> | 58.854 | 10.07 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 8.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 7 | 298 | 22% monomethyl polyethylene glycol 5000, 100 mM MOPS pH 7.0, 25 mM MgCl2, 2% 2,2,2-trifluoroethanol, 6% glycerol, Batch, temperature 298K |
