4NXW

Crystal structure of the cytosolic domain of human MiD51

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.45860
Spacegroup name	P 43 21 2
Unit cell lengths	57.534, 57.534, 253.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.617 - 2.550
Rwork	0.197
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.005
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.083	0.466
Number of reflections	14827
<I/σ(I)>	28.7	7.7
Completeness [%]	100.0	100
Redundancy	22.3	23.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.0M sodium malonate, 0.01M ADP, 0.01M manganese chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K