4NX0
Crystal structure of Abp-WT, a GH27-b-L-arabinopyranosidase from Geobacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.954 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.708, 202.156, 287.289 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.280 |
R-factor | 0.14722 |
Rwork | 0.146 |
R-free | 0.17671 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cc1 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.892 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.073 | 0.073 |
Number of reflections | 261171 | |
<I/σ(I)> | 9.6 | |
Completeness [%] | 96.7 | 96.7 |
Redundancy | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.8M ammonium sulfate, 0.1M citrate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |