4NWW

Crystal structure of an N-terminally truncated capsid protein mutant of Orsay virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	I 2 2 2
Unit cell lengths	404.921, 375.148, 412.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.750
Rwork	0.299
R-free	0.30080
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.810
High resolution limit [Å]	3.750	10.060	3.750
Rmerge	0.247	0.052	0.668
Number of reflections	204023
<I/σ(I)>	2.8
Completeness [%]	64.3	54.6	68
Redundancy	1.9	2.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.7-1.05 M sodium malonate, 0.25%-1% (v/v) Jeffamine ED-2001, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K