4NWI
Crystal structure of cytosolic 5'-nucleotidase IIIB (cN-IIIB) bound to cytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.600, 99.350, 72.810 |
| Unit cell angles | 90.00, 92.46, 90.00 |
Refinement procedure
| Resolution | 35.875 - 2.050 |
| R-factor | 0.2284 |
| Rwork | 0.228 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nv0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.818 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.880 | 2.150 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.083 | 0.492 |
| Number of reflections | 40144 | |
| <I/σ(I)> | 11.07 | 2.37 |
| Completeness [%] | 96.5 | 97.1 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 26% PEG 4000, 0.2M NaCl, 0.1M HEPES , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
