4NWH
Lysozyme UNDER 30 BAR PRESSURE OF XENON
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2011-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980590 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.306, 79.306, 38.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.17512 |
| Rwork | 0.173 |
| R-free | 0.20816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c10 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.080 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 15059 | |
| <I/σ(I)> | 17.7 | 7.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 4.6 | 300 | 0.1M sodium acetate, 0.6-1.1M NaCl, pH 4.6, BATCH, temperature 300K |
