4NVS
Crystal Structure of the Q18CP6_CLOD6 protein from glyoxalase family. Northeast Structural Genomics Consortium Target CfR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.388, 52.168, 135.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.176 - 2.385 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.28300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.101 | 0.499 |
| Number of reflections | 27413 | |
| <I/σ(I)> | 22.7 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 5.8 | 291 | 27% PEG 3350, 5% 1-ethyl-3-methylimidazolium chloride, 0.1 M MES pH 5.8, Microbatch crystallization under oil, temperature 291K |
