4NV8
Crystal Structure of Mesorhizobium Loti Arylamine N-acetyltransferase F42W Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Detector technology | PIXEL |
| Collection date | 2013-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07169 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.860, 115.080, 115.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.010 - 1.840 |
| R-factor | 0.20486 |
| Rwork | 0.204 |
| R-free | 0.22807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.451 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.010 | 1.890 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.064 | 0.601 |
| Number of reflections | 61856 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG 5000, 0.2M ammonium sulfate, 0.1M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
