4NV7
Crystal Structure of Mesorhizobium Loti Arylamine N-acetyltransferase 1 In Complex With CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Detector technology | PIXEL |
| Collection date | 2013-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.500, 114.860, 115.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.180 - 2.020 |
| R-factor | 0.19458 |
| Rwork | 0.193 |
| R-free | 0.22076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.323 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.540 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.095 | 0.636 |
| Number of reflections | 47549 | |
| <I/σ(I)> | 12 | 2.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Crystallization details: Should be 30% PEG 5000, 0.2M ammonium sulfate, 0.1M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
