4NV5
C50A mutant of Synechococcus VKOR, C2 crystal form (dehydrated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-04-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.842, 118.822, 68.034 |
| Unit cell angles | 90.00, 89.85, 90.00 |
Refinement procedure
| Resolution | 37.800 - 2.790 |
| R-factor | 0.23355 |
| Rwork | 0.232 |
| R-free | 0.24516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PARROT |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.078 | 0.762 |
| Number of reflections | 27546 | |
| <I/σ(I)> | 15.2 | 1.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 12% PEG3350, 0.1M sodium cacodylate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
