4NUV
Heterotetramer structure of Region II from Plasmodium vivax Duffy Binding Protein (PvDBP) bound to the ectodomain of the Duffy Antigen Receptor for Chemokines (DARC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-12-20 |
| Detector | NOIR-1 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.287, 59.391, 91.395 |
| Unit cell angles | 103.14, 91.04, 100.31 |
Refinement procedure
| Resolution | 19.904 - 2.600 |
| R-factor | 0.1854 |
| Rwork | 0.183 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rrc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 22139 | |
| Completeness [%] | 95.2 | 93.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 290 | 0.1 M HEPES and 20% (w/v) polyethylene glycol 6000, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 7.4 |
