4NUO
Crystal structure of zinc-bound Na-ASP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95375 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.051, 51.477, 43.139 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 28.900 - 1.750 |
| R-factor | 0.1654 |
| Rwork | 0.164 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nui |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 31306 | |
| Completeness [%] | 97.7 | 90 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 34% PEG 4000, 20mM MOPS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
