4NU3
Crystal structure of mFfIBP, a capping head region swapped mutant of ice-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.681, 51.459, 75.230 |
| Unit cell angles | 104.41, 96.85, 97.67 |
Refinement procedure
| Resolution | 49.110 - 1.399 |
| R-factor | 0.22454 |
| Rwork | 0.223 |
| R-free | 0.25016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nu2 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 1.420 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 78787 | |
| Completeness [%] | 93.2 | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30%(w/v) PEG4000, 0.19M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
