4NTT
Structure of the catalytic subunit of cAMP-dependent protein kinase bound to ADP and one magnesium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Detector technology | CCD |
| Collection date | 2012-03-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.480, 143.150, 62.570 |
| Unit cell angles | 90.00, 103.61, 90.00 |
Refinement procedure
| Resolution | 71.580 - 3.500 |
| R-factor | 0.23015 |
| Rwork | 0.227 |
| R-free | 0.28371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nts |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.150 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 10660 |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 8% MPD, 0.1 M Bicine, 150 mM Ammonium Acetate, 10 mM DTT, 9% methanol added to the well, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
