4NTA
Mus Musculus LTC4 synthase in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 169.575, 169.575, 169.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.394 - 2.700 |
| R-factor | 0.2302 |
| Rwork | 0.229 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uui |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.284 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.959 | 42.394 | 2.850 |
| High resolution limit [Å] | 1.999 | 8.540 | 2.700 |
| Rmerge | 0.030 | 0.817 | |
| Total number of observations | 1209 | 4643 | |
| Number of reflections | 11165 | ||
| <I/σ(I)> | 14 | 8.2 | 0.9 |
| Completeness [%] | 99.5 | 98.5 | 99 |
| Redundancy | 3.3 | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 298 | 1.9 M NH4SO4, 0.2 M NaCl, 0.1 M Na-cacadylate, pH 6.3, VAPOR DIFFUSION, temperature 298K |
