4NT4
Crystal structure of the kinase domain of Gilgamesh isoform I from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.025, 52.025, 291.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.790 - 2.860 |
| R-factor | 0.258 |
| Rwork | 0.254 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2izr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.751 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 9124 | |
| <I/σ(I)> | 16.2 | 4.5 |
| Completeness [%] | 90.7 | 100 |
| Redundancy | 7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | 1% trypton, 50mM HEPES pH 7.0, 9% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
