4NSE
BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE, H4B-FREE, L-ARG COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.660, 106.100, 155.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.970 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.054 * | 0.660 * |
| Total number of observations | 255850 * | |
| Number of reflections | 69580 | |
| <I/σ(I)> | 9.6 | 1.7 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 15 (%) | |
| 2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 4 | 1 | drop | SEITU | 1 (mM) | can be replaced by 5mM L-Arg |
| 5 | 1 | drop | SDS | 0.035 (mM) | |
| 6 | 1 | drop | TCEP | 5 (mM) | can be replaced by 5mM glutathione sulfonate |
