4NSC
Crystal Structure of CBARA1 in the Apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.879, 146.819, 115.872 |
| Unit cell angles | 90.00, 111.08, 90.00 |
Refinement procedure
| Resolution | 36.720 - 3.200 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.30700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.7) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 44146 | |
| Completeness [%] | 94.6 | 69 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 8% PEG 3350 and 0.075 M ammonium citrate tribasic pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
