4NS5

Crystal structure of human BS69 Bromo-Zinc finger-PWWP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-22
Detector	RAYONIX MX-225
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	54.760, 64.380, 73.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.880 - 1.900
R-factor	0.2087
Rwork	0.206
R-free	0.25060
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.128
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	SOLVE (2.13)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.400	31.883	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.069	0.548
Total number of observations		4834	21967
Number of reflections	20832
<I/σ(I)>	12.2	7.1	1.3
Completeness [%]	99.1	98.9	98.4
Redundancy	7.3	6.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	285	0.1M Tris-HCl pH 9.0, 4% w/v Polyethylene glycol (PEG) 8000, VAPOR DIFFUSION, SITTING DROP, temperature 285K
2	VAPOR DIFFUSION, SITTING DROP	8.4	285	0.1 M Bicine, pH8.4, 2% v/v 1, 4-Dioxane, 2% w/v PEG 20000, VAPOR DIFFUSION, SITTING DROP, temperature 285K