4NRW
MvNei1-G86D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.401, 121.682, 79.993 |
| Unit cell angles | 90.00, 95.68, 90.00 |
Refinement procedure
| Resolution | 14.966 - 2.845 |
| R-factor | 0.2653 |
| Rwork | 0.260 |
| R-free | 0.31450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a46 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.614 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 | 2.950 |
| High resolution limit [Å] | 2.845 | 6.020 | 2.845 |
| Rmerge | 0.185 | 0.114 | 0.523 |
| Number of reflections | 17340 | ||
| <I/σ(I)> | 4.6 | ||
| Completeness [%] | 99.0 | 99.3 | 98.7 |
| Redundancy | 3.5 | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 285 | 4% w/v PEG8000, 0.125 M potassium chloride, 0.05 M magnesium sulfate, 100 mM MES, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
