4NRN
Crystal structure of metal-bound toxin from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Detector technology | CCD |
| Collection date | 2011-11-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.012, 47.028, 52.599 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 21.876 - 1.802 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ls4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.096 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 14014 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | polyethylene glycol 3350, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
