4NQV
Crystal Structure of HLA A*0101 in complex with NP44, an 9-mer influenza epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 265.538, 81.496, 140.071 |
| Unit cell angles | 90.00, 121.58, 90.00 |
Refinement procedure
| Resolution | 43.490 - 2.390 |
| R-factor | 0.2573 |
| Rwork | 0.256 |
| R-free | 0.29140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bo8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.492 |
| High resolution limit [Å] | 2.390 |
| Number of reflections | 100231 |
| <I/σ(I)> | 10.7 |
| Completeness [%] | 99.0 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 277 | 0.2M MgCl, 0.1M Na-citrate pH 5.6, 15-20% PEG 6K, EVAPORATION, temperature 277K |
