4NQQ
Crystal structure of mouse P-cadherin extracellular domains EC1-EC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.085, 188.700, 53.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qvi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.896 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1186)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.589 | |
| Number of reflections | 20958 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.3 | 97.3 |
| Redundancy | 6.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 38% PEG 6000, Bis-Tris pH 6.5, 0.35M Calcium chloride, 0.01M Copper (II) chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
