4NQJ
Structure of coiled-coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-06 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.097, 101.064, 233.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.139 - 2.152 |
| R-factor | 0.2179 |
| Rwork | 0.216 |
| R-free | 0.25050 |
| Starting model (for MR) | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 52974 | |
| Completeness [%] | 100.0 | 98.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
