4NQF
Crystal structure of HEPN domain protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.103, 71.574, 83.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.040 - 2.700 |
| R-factor | 0.22395 |
| Rwork | 0.218 |
| R-free | 0.28005 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.372 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | Phenix-AutoSol |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.040 | 36.040 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | |
| Number of reflections | 10678 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% Polyethylene glycol 3350, 100mM Imidazole, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
