4NQ0
Structural insights into yeast histone chaperone Hif1: a scaffold protein recruiting protein complexes to core histones
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.308, 78.280, 82.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.300 - 2.100 |
R-factor | 0.21269 |
Rwork | 0.211 |
R-free | 0.24304 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.001 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.083 | 0.656 |
Number of reflections | 18861 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 99.6 | 99 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 287 | 0.1M sodium cacodylate, 15% PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |