4NPO

Crystal structure of protein with unknown function from Deinococcus radiodurans at P61 spacegroup

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	KURCHATOV SNC BEAMLINE K4.4
Synchrotron site	KURCHATOV SNC
Beamline	K4.4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-02
Detector	MAR CCD 165 mm
Wavelength(s)	0.9815
Spacegroup name	P 61
Unit cell lengths	80.390, 80.390, 69.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.370 - 1.470
R-factor	0.15094
Rwork	0.150
R-free	0.17462
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e8o
RMSD bond length	0.019
RMSD bond angle	1.901
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.470	1.470
Number of reflections	43293
Completeness [%]	98.0	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.5M Sodium chloride, 10mM HEPES, 0.1M Sodium acetate, 2.6M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K