4NPH
Crystal structure of SsaN from Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.138, 76.312, 39.094 |
| Unit cell angles | 90.00, 103.71, 90.00 |
Refinement procedure
| Resolution | 37.028 - 2.090 |
| R-factor | 0.1947 |
| Rwork | 0.191 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2obm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((AutoMR)) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.514 |
| Number of reflections | 22889 | |
| <I/σ(I)> | 11.09 | 2.6 |
| Completeness [%] | 98.1 | 98.5 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, 10% v/v jeffamine M-600, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
