4NPA
Scrystal structure of protein with unknown function from Vibrio cholerae at P22121 spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-24 |
| Detector | MAR225HE |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.330, 96.340, 333.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 2.100 |
| R-factor | 0.20941 |
| Rwork | 0.206 |
| R-free | 0.26674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pmb |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 111393 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 500mM NaCl, 10mM HEPES, 4%(v/v) Tacsimate, 18%(w/v) PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
