4NP9
Structure of Rabphilin C2A domain bound to IP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-03-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.270, 39.470, 89.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.566 - 1.920 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.930 | 2.041 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.087 | 0.300 |
| Number of reflections | 10862 | |
| <I/σ(I)> | 13.19 | 4.2 |
| Completeness [%] | 98.7 | 95 |
| Redundancy | 9.5 | 50.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 25-28% PEG 4K, 10-20mM Ammonium sulfate, 100mM HEPES pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
