4NP6
Crystal Structure of Adenylate Kinase from Vibrio cholerae O1 biovar eltor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 43 |
| Unit cell lengths | 101.617, 101.617, 89.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.445 - 2.004 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | SAD and Molecular replacement |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 61011 | |
| <I/σ(I)> | 8.7 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 1.4 M sodium citrate tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
