4NP5
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature
Replaces: 4EOAExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.221, 60.453, 38.536 |
Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
Resolution | 38.450 - 1.500 |
R-factor | 0.18225 |
Rwork | 0.180 |
R-free | 0.21446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.019 |
RMSD bond angle | 1.942 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.062 | 0.056 | 0.155 |
Number of reflections | 22825 | ||
<I/σ(I)> | 16.4 | 16.3 | |
Completeness [%] | 99.7 | 99.9 | 98.9 |
Redundancy | 9.2 | 8.4 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |