4NOY

Crystal structure of Listeria monocytogenes Hfq F43W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 2 21
Unit cell lengths	63.957, 66.967, 106.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.425 - 2.795
R-factor	0.2298
Rwork	0.227
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nl2
RMSD bond length	0.014
RMSD bond angle	1.464
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.967	50.000	2.850
High resolution limit [Å]	2.795	7.590	2.795
Rmerge	0.124	0.059	0.384
Number of reflections	11574
<I/σ(I)>	8.8
Completeness [%]	98.5	96.4	95.8
Redundancy	4.1	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	40% 1,2-propanediol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K