4NOI

2.17 Angstrom Crystal Structure of DNA-directed RNA Polymerase Subunit Alpha from Campylobacter jejuni.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	99.173, 62.743, 93.621
Unit cell angles	90.00, 112.52, 90.00

Refinement procedure

Resolution	29.490 - 2.170
R-factor	0.1762
Rwork	0.174
R-free	0.22505
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.521
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	REFMAC (5.8.0046)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.210
High resolution limit [Å]	2.170	2.170
Rmerge	0.077	0.566
Number of reflections	54356
<I/σ(I)>	21.1	2.1
Completeness [%]	99.4	100
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	Protein: 23 mG/mL, 0.25 M Sodium chloride, 0.01 M Tris-HCL buffer pH 8.3; Screen: PACT (F3), 0.2M Sodium iodine, 0.1M Bis-Tris propane pH 6.5, 20% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K