4NOF
Crystal structure of the second Ig domain from mouse Polymeric Immunoglobulin receptor [PSI-NYSGRC-006220]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.388, 35.365, 73.238 |
| Unit cell angles | 90.00, 105.96, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly-alanine model of PDB code 1XED |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.531 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.082 | 0.702 |
| Number of reflections | 30181 | |
| <I/σ(I)> | 18.3 | 1.89 |
| Completeness [%] | 98.6 | 94 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 5% glycerol), Reservoir (0.1 M Citric Acid:NaOH pH 3.5, 25% (w/v) PEG 3350), Cryoprotection (30% Glycerol), Vapor Diffusion, Sitting Drop, temperature 298K |
