4NOC
The crystal structure of a CBS Domain-containing Protein of Unknown Function from Kribbella flavida DSM 17836.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97929, 0.97945 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 99.434, 99.434, 260.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.522 - 2.300 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.23980 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.619 |
| Number of reflections | 29395 | |
| <I/σ(I)> | 36.1 | 3.2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 1.6M Ammomnium Sulfate (diabasic), 0.1M Sodium acetate/Acetate acid and 0.2M Sodium Chloride., pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
