4NOA
Truncated minor pilin PilE from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.159, 35.560, 43.536 |
Unit cell angles | 90.00, 97.33, 90.00 |
Refinement procedure
Resolution | 43.181 - 1.250 |
R-factor | 0.1601 |
Rwork | 0.159 |
R-free | 0.17530 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.965 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.084 | 0.210 |
Number of reflections | 30749 | |
<I/σ(I)> | 20.7 | 7.6 |
Completeness [%] | 95.4 | 89.6 |
Redundancy | 6.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium tartrate dibasic, 20% (w/v) PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |