4NNZ
Subunit PA0372 of heterodimeric zinc protease PA0371-PA0372
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-25 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.880, 106.510, 112.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.400 - 2.478 |
| R-factor | 0.2029 |
| Rwork | 0.202 |
| R-free | 0.22704 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.661 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.400 | 50.000 |
| High resolution limit [Å] | 2.478 | 2.478 |
| Number of reflections | 34553 | |
| Completeness [%] | 99.1 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 298 | 15% PEG 4000, 0.1M Imidazole Malonate, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
