4NNQ
Crystal structure of LnmF protein from Streptomyces amphibiosporus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97933 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 104.148, 104.983, 123.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.060 - 2.010 |
| R-factor | 0.1849 |
| Rwork | 0.184 |
| R-free | 0.22720 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.040 |
| High resolution limit [Å] | 2.010 | 5.450 | 2.010 |
| Rmerge | 0.098 | 0.054 | 0.897 |
| Number of reflections | 45121 | ||
| <I/σ(I)> | 18 | 1.72 | |
| Completeness [%] | 99.8 | 97.3 | 100 |
| Redundancy | 5.8 | 5.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 0.2 M NaCl, 0.1 M CHES, 1.26 M (NH4)2SO4, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
