4NN2

Protein Crystal Structure of Human Borjeson-Forssman-Lehmann Syndrome Associated Protein PHF6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 3 2 1
Unit cell lengths	102.780, 102.780, 51.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.290 - 1.472
R-factor	0.1597
Rwork	0.159
R-free	0.17640
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.128
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	89.010	36.289	1.550
High resolution limit [Å]	1.472	4.650	1.470
Rmerge	0.092	0.034	0.505
Total number of observations		9796	34945
Number of reflections	52631
<I/σ(I)>	10.2	16.7	1.3
Completeness [%]	99.4	98.2	99.8
Redundancy	5	5.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	0.2M amminium acetate, 0.1M tri-sodium citrate dehydrate, 30% (w/v) PEG4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K