4NMI
Crystal Structure of the Apo ectoine hydroxylase ECTD from Salibacillus salexigens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2013-08-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.897, 102.897, 159.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.780 |
| R-factor | 0.17302 |
| Rwork | 0.172 |
| R-free | 0.19973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.042 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.826 |
| High resolution limit [Å] | 1.700 | 1.780 |
| Number of reflections | 37155 | |
| Completeness [%] | 92.8 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 100 mM MES pH 5.0 and 1.2 M ammonium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
