4NM4
Crystal structure of broadly neutralizing antibody CR8043
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-06-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.954, 68.634, 72.401 |
| Unit cell angles | 78.67, 78.93, 86.18 |
Refinement procedure
| Resolution | 44.447 - 2.650 |
| R-factor | 0.1781 |
| Rwork | 0.175 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vge |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.086 | 0.515 |
| Number of reflections | 31483 | |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 97.1 | 93.8 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 20% PEG 6000, 0.1 M HEPES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
