4NLX

Poliovirus Polymerase - G289A/C290V Loop Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-08
Detector	NOIR-1
Wavelength(s)	1.000
Spacegroup name	P 65
Unit cell lengths	129.177, 129.177, 111.613
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.600
Rwork	0.208
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	d*TREK (9.4L)
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.890	29.890	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.093	0.039	0.311
Total number of observations		25182	24069
Number of reflections	61925
<I/σ(I)>	14	30.1	5.5
Completeness [%]	99.2	97	99.8
Redundancy	7.55	7.52	7.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K